Global Academic Research Institute (Pvt) Ltd was founded in 2010 with the purpose of becoming the leading conference organizer in Sri Lanka. We have organized several International Conferences all around the world. Even though we established recently, we were able to come to this position because of the spirit of our GARI conference team members and their creative and innovative ideas.

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2  Pseudomonas fluorescensas the causative agent of blue coloration in fresh cheese

The aim of this paper was to identify the source of microbiological contamination and the bacteriological cause of a unique spoilage pattern, characterized by a distinct blue discoloration of fresh cheese, which occurred in farm cheese from Gran Canaria (Canary Islands, Spain). The genus Pseudomonas are important medically because they areconsidered opportunistic pathogens for humans and animals while others, like phytopathogens, are very important in the agricultural sector (Ridgway and Safarik, 1990). Thus, we studied it using traditional microbiological methods and genotypic tests. These techniques identified Pseudomonas fluorescensbiovar IV, which produces a blue, non-diffusible pigment, as the likely contaminating agent. The isolates collected from contaminated cheese, milk, water and curd were initially identified by the Analytical Profile Index test as P. aeruginosa and later confirmed by 16S rDNA sequencing as Pseudomonas fluorescens. Pulsed field gel electrophoresis with the enzyme XbaI identified Pseudomonas fluorescens in milk and cheese. Pasteurizer installation and a new cleaning-disinfection program allowed us to control Pseudomonas spp. until all the blue coloration in the cheese had been eliminated. Our data suggested that the source of contamination could have been due to the use of non-chlorinated water of uncertain origin in the dairy facilities and on the farm.

Keywords: fresh cheese; Pseudomonas fluorescens; food safety

Posted by Prof.Antonio Jose de Almeida Simoes Raposo
Best paper award for:Prof.Antonio Jose de Almeida Simoes Raposo Campus Universitario,
Portugal

1  Why Vi negative isolates of Salmonella enterica serovar Typhi are significantly present in some regions and not in others?

The emergence of Vi negative isolates of Salmonella enterica serovar Typhi (S. Typhi) in recent years has sparked a lot of interest because these isolates have direct implications on future development of typhoid vaccines which currently are almost exclusively Vi polysaccharide based. Initially it was thought that Vi negativity may actually be due to storage under artificial conditions but now it has been shown that Vi negative isolates of S.

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Posted by Prof Dr. Abdul Haque, PhD, Department of Pathology, The University of Faisalabad,Faisalabad, Pakistan

2  Pseudomonas fluorescensas the causative agent of blue coloration in fresh cheese

The aim of this paper was to identify the source of microbiological contamination and the bacteriological cause of a unique spoilage pattern, characterized by a distinct blue discoloration of fresh cheese, which occurred in farm cheese from Gran Canaria (Canary Islands, Spain).

Read More 

Posted by Prof.Antonio Jose de Almeida Simoes Raposo
Best paper award for:Prof.Antonio Jose de Almeida Simoes Raposo Campus Universitario,
Portugal